This report describes a setup for the downward capillary blotting of RNA with the use Nike Free Mens Uk of 10 × Nike Free Trainer 5.0 Uk
SSC as a transfer solution. The setup is composed of a stack of blotting papers, hybridization membrane, and agarose gel. Two layers of blotting paper connect the stack with two reservoirs containing transfer solution. Using this setup, blotting of RNA fragments (<7.5 kb) can be completed in 1 h. If necessary, the blotting time can be expanded from 1 to 18 h without decrease in hybridization efficiency of RNA.
Interest in stakeholder approaches to strategic management is growing around the world but at the same time top management's concern with shareholder value has never been greater. Managers in all kinds of firms are faced with the dilemma of how to satisfy the competing claims of shareholders and the other stakeholders. The
consequences are evident everywhere as shareholders grow rich and unemployment increases. This article examines the stakeholder theory and its potential for solving management's dilemma.
Groundwater samples were collected from stock wells and bores during September 1979, June 1980, November 1980 and November 1981, and were analyzed for uranium by XRF and fluorimetry. It was found that: (1) High U concentrations were generally confined to the immediate vicinity of granitoid bodies; and (2) with the exception of two open, shallow wells, concentrations of U did not vary more than ±25 μg l−1 over the sampling period, despite very heavy rainfall (rainfall events > 100 mm day−1) in 1980.